Is there any change in chlorophyll content in leaves and washed leaves?

Is there any change in chlorophyll content in leaves and washed leaves?
Nitrogen is the most obvious nutrient for tea growth, quality and yield. Appropriate application of nitrogen can not only increase crop yield but also appropriately increase crop quality. However, excessive application of nitrogen fertilizer can not only increase crop side yield. , but also will reduce the quality of the crop and pollute the environment. Therefore, the inspection of nutrients for crops, understanding of the key period of fertilizer requirements, and timely and quantitative supply of nutrients can effectively increase the economic benefits of fertilization. However, using nitrogen analysis method to understand nitrogen is not only cumbersome, but also time consuming and time-consuming. The use of chlorophyll meter to measure and analyze the nitrogen of plants can avoid the above problems.
When measuring with a chlorophyll meter, what are the factors that affect the accuracy of the padding? There is no doubt that the variety of the crop and the measured leaf position will affect the padding value. So what other factors will affect its accuracy? Through two aspects to compare, respectively: the impact of isolated leaves on chlorophyll content, whether there is a change in leaf chlorophyll content after cleaning.
The detection and operation method of isolated leaves are as follows: The handheld blade chlorophyll meter is used to measure and record the data, and then the blades are collected and brought back for 1, 4, 8 hours to use portable chlorophyll. The analyzer was used to measure the data. It was found through the data that the chlorophyll content of the isolated leaves changed little.
After cleaning the blades, the operation was measured before cleaning, and then measured after cleaning. The data was analyzed twice. It was found that the cleaned chlorophyll detector readings were slightly reduced (within one unit) or remained unchanged. The test showed no significant difference in the measured values. The reduced reading of SPAD after washing may be related to changes in SPAD readings caused by dust on the leaves.

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